The Mouse Interleukin-9 (IL-9) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of interleukin-9 in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.Interleukin-9 is a key cytokine that plays a vital role in regulating immune responses and inflammation. It has been implicated in a range of diseases, including asthma, autoimmune disorders, and cancer.
By measuring IL-9 levels, researchers can gain valuable insights into disease mechanisms and potentially discover new therapeutic targets.With the Mouse Interleukin-9 (IL-9) ELISA Kit from Assay Genie, researchers can confidently study the role of IL-9 in various disease models and advance their research efforts towards developing novel treatment strategies.
Product Name:
Mouse Interleukin 9 (IL-9) ELISA Kit
Product Code:
MODL00719
Size:
96 Assays
Target:
IL9
Synonyms:
HP40, P40, TCGFIII, TCGF3, T Cell Growth Factor III, P40 T-Cell And Mast Cell Growth Factor, P40 Cytokine, Homolog Of Mouse T Cell And Mast Cell Growth Factor 40
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
