The Mouse Irisin ELISA Kit is designed for the precise quantification of Irisin levels in various biological samples obtained from mouse models. Irisin is a myokine derived from skeletal muscle that plays a significant role in regulating energy metabolism, thermogenesis, and exercise-related benefits in mice. Understanding the levels of Irisin can provide valuable insights into metabolic adaptations, exercise responses, and metabolic disorders in mouse studies. Assay Genie's Mouse Irisin ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control guidelines, this kit delivers robust performance and is user-friendly, making it an ideal choice for researchers studying metabolism, energy expenditure, and exercise physiology in mouse models. Trust Assay Genie's Mouse Irisin ELISA Kit for reliable quantification of Irisin levels in your research endeavors.
Product Name:
Mouse Irisin ELISA Kit
SKU:
AEES00197
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
