Mouse JNK1/MAPK8 Associated Membrane Protein (JKAMP) ELISA Kit
The Mouse JNK1/MAPK8-Associated Membrane Protein (JKAMP) ELISA Kit from AssayGenie is specifically designed for the precise detection of JKAMP levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and selectivity, ensuring accurate and consistent results for a variety of research applications.JKAMP is a critical protein that interacts with JNK1/MAPK8, playing a key role in various cellular processes including cell signaling, proliferation, and apoptosis.
Dysregulation of JKAMP has been implicated in several diseases such as cancer, inflammatory disorders, and neurodegenerative conditions, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.Overall, the Mouse JNK1/MAPK8-Associated Membrane Protein (JKAMP) ELISA Kit provides researchers with a reliable tool for studying the function and regulation of JKAMP in biological systems, offering valuable insights into its role in health and disease.
Product Name:
Mouse JNK1/MAPK8 Associated Membrane Protein (JKAMP) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
