Mouse LCN2 Recombinant Protein (N-His) (GXRP05825)

Mouse LCN2 Recombinant Protein (N-His)

Mouse LCN2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asp41-Asn200, with an N-terminal His tag. Predicted molecular weight: 21.05 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Neutrophil gelatinase-associated lipocalin (LCN2/NGAL) is a ~22 kDa protein. Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. For research use only.

Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis.