The Mouse LEP (Leptin) ELISA Kit is a specialized assay designed for the precise quantitative measurement of leptin levels in various biological samples derived from mouse models. Leptin is a crucial hormone known for its role in regulating energy balance, metabolism, and adipose tissue development. It serves as a key factor in appetite control, energy expenditure, and the maintenance of body weight. With Assay Genie's Mouse LEP ELISA Kit, researchers can achieve reliable and reproducible results due to its exceptional sensitivity and specificity. This kit offers a high-quality and efficient solution for studying leptin levels, providing valuable insights into metabolic functions, obesity-related mechanisms, and potential therapeutic strategies. Trust in the accuracy and performance of Assay Genie's Mouse LEP ELISA Kit for your research needs, ensuring a robust tool for investigating the intricate role of leptin in metabolic regulation and related physiological processes.
Product Name:
Mouse LEP (Leptin) ELISA Kit
SKU:
AEES00203
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
