Mouse Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit
Based on the information provided in the URL, the Mouse Macrophage Inflammatory Protein-3 Beta (MIP-3B) ELISA Kit is a specialized tool designed for the accurate quantification of MIP-3B levels in mouse serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, guaranteeing precise and consistent results for a variety of research applications.MIP-3B is a key chemokine involved in the regulation of inflammation and immune responses, playing a crucial role in recruiting immune cells to sites of infection or tissue injury.
Dysregulation of MIP-3B levels has been linked to various inflammatory conditions, autoimmune diseases, and cancer, making it a valuable biomarker for understanding these diseases and exploring potential therapeutic interventions.Overall, the Mouse Macrophage Inflammatory Protein-3 Beta (MIP-3B) ELISA Kit offers researchers a reliable and efficient tool for investigating the role of MIP-3B in health and disease, providing valuable insights into the mechanisms underlying inflammation and immune system function.
Product Name:
Mouse Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
