The Mouse MHC I (H-2Ld) Major Histocompatibility Complex 1 CLIA Kit (MOES00432) is a specialized assay kit designed for the quantitative measurement of MHC I (H-2Ld) levels in mouse samples. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.MHC I (H-2Ld) is a key component of the mouse immune system, playing a critical role in antigen presentation and immune response. Monitoring MHC I levels can provide valuable insights into immune function, infection, and disease progression in mouse models.
Ideal for use with serum, plasma, and cell culture supernatants, the Mouse MHC I (H-2Ld) Major Histocompatibility Complex 1 CLIA Kit (MOES00432) is a valuable tool for researchers studying immunology, infectious diseases, and autoimmunity in mouse models. Purchase this kit today to advance your research and gain a deeper understanding of immune responses in mice.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MHCⅠ/H-2Ⅰ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MHCⅠ/H-2Ⅰ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MHCⅠ/H-2Ⅰ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MHCⅠ/H-2Ⅰ. You can calculate the concentration of Mouse MHCⅠ/H-2Ⅰ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-101
100-117
91-106
Average (%)
96
107
98
1:4
Range (%)
100-112
89-104
93-109
Average (%)
106
95
99
1:8
Range (%)
94-107
101-114
100-114
Average (%)
99
108
107
1:16
Range (%)
95-110
102-116
98-112
Average (%)
103
109
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.88
26.07
90.28
9.28
28.38
91.72
Standard deviation
1.13
2.24
9.66
0.99
2.84
7.03
C V (%)
11.44
8.59
10.7
10.67
10.01
7.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MHCⅠ/H-2Ⅰ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MHCⅠ/H-2Ⅰ in samples. No significant cross-reactivity or interference between Mouse MHCⅠ/H-2Ⅰ and analogues was observed.
