Description
Mouse MITF Recombinant Protein (N-His)
Mouse MITF Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser50-Arg400, with an N-terminal His tag. Predicted molecular weight: 42.23 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Microphthalmia-associated transcription factor is a ~58 kDa protein. Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis. For research use only.
Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR). Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity. Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1).
| Product Name: | Mouse MITF Recombinant Protein (N-His) |
| SKU: | GXRP09839 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mouse |
| Synonyms: | Microphthalmia-associated transcription factor; Mitf; Bw; Mi; Vit |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser50-Arg400 |
| Calculated MW: | 42.23 kDa |
| UniProt: | Q08874 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
