The Mouse NF-kB p65 (Nuclear Factor kB p65) ELISA Kit is specifically designed for the accurate quantification of NF-kB p65 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.NF-kB p65 is a key transcription factor involved in regulating the expression of genes related to inflammation, immune responses, and cell survival. Dysregulation of NF-kB p65 has been linked to various diseases, including cancer, inflammatory disorders, and autoimmune conditions.
Therefore, this ELISA kit is essential for studying NF-kB p65 signaling pathways and potential therapeutic interventions.Overall, the Mouse NF-kB p65 ELISA Kit from Assay Genie provides researchers with a valuable tool for investigating the role of NF-kB p65 in health and disease, offering accurate and precise measurements for insightful research discoveries.
Product Name:
Mouse NF-kB p65 (Nuclear Factor-kB p65) ELISA Kit
SKU:
MOES01305
Target:
Mouse NF-kB p65 (Nuclear Factor-kB p65)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NFKB-p65. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NFKB-p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NFKB-p65. You can calculate the concentration of Mouse NFKB-p65 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
98-110
93-107
Average (%)
97
104
101
1:4
Range (%)
88-102
86-98
83-97
Average (%)
93
92
88
1:8
Range (%)
88-104
85-97
86-98
Average (%)
96
90
91
1:16
Range (%)
90-102
83-93
84-96
Average (%)
96
88
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
1.94
6.71
1.15
2.11
6.27
Standard deviation
0.05
0.1
0.3
0.06
0.12
0.22
C V (%)
4.76
5.15
4.47
5.22
5.69
3.51
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NFKB-p65 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NFKB-p65 in samples. No significant cross-reactivity or interference between Mouse NFKB-p65 and analogues was observed.
