Human NF-kB p65 (Nuclear Factor Kappa B p65) ELISA Kit
The Human NF-κB p65 (Nuclear Factor Kappa B p65) ELISA Kit is a powerful tool designed for the precise measurement of NF-κB p65 levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a diverse range of research applications.NF-κB p65 is a key transcription factor that plays a critical role in regulating immune responses, inflammation, and cell survival. Dysregulation of NF-κB signaling has been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory conditions. As such, the measurement of NF-κB p65 levels can provide valuable insights into disease pathology and guide the development of targeted therapeutics.With its user-friendly protocol and robust performance, the Human NF-κB p65 ELISA Kit is an indispensable tool for researchers seeking to better understand the molecular mechanisms underlying NF-κB signaling and its implications in health and disease. Trust in this kit to deliver reliable and reproducible data, paving the way for groundbreaking discoveries in the field of biomedical research.
Product Name:
Human NF-kB p65 (Nuclear Factor Kappa B p65) ELISA Kit
SKU:
HUES02431
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
