Human NF-kB p65 (Nuclear Factor Kappa B p65) ELISA Kit (HUES02431)
- SKU:
- HUES02431
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- Q04206
- Sensitivity:
- 0.09ng/mL
- Range:
- 0.16-10ng/mL
- ELISA Type:
- Sandwich
- Synonyms:
- NFKB3, p65, RelA
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Immunology
Description
Product Name: | Human NF-kB p65 (Nuclear Factor Kappa B p65) ELISA Kit |
SKU: | HUES02431 |
Target: | Human NF-kB p65 (Nuclear Factor Kappa B p65) |
Size: | 96T |
Assay type: | Sandwich-ELISA |
Assay time: | 3.5h |
Sensitivity: | 0.10 ng/mL |
Detection range: | 0.16-10 ng/mL |
Kit component: |
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This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NFKB-p65. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NFKB-p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NFKB-p65. You can calculate the concentration of Human NFKB-p65 in the samples by comparing the OD of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | serum, plasma and other biological fluids; 100μL |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of Human NFKB-p65 concentrations in serum, plasma and other biological fluids. |
Specificity: | This kit recognizes Human NFKB-p65 in samples. No significant cross-reactivity or interference between Human NFKB-p65 and analogues was observed. |