SynonymsSLC6A2,NAT1,NET,NET1,Sodium-dependent noradrenaline transporter,Noradrenaline Transporter,Solute Carrier Family 6 member 2,Neurotransmitter transporter,Noradrenalin
ReactivityMouse
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
MODL00932
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O55192
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC6A2,NAT1,NET,NET1,Sodium-dependent noradrenaline transporter,Noradrenaline Transporter,Solute Carrier Family 6 member 2,Neurotransmitter transporter,Noradrenalin
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Mouse Norepinephrine Transporter (NET) ELISA Kit is an essential tool for accurately measuring levels of NET in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing dependable and consistent results for a variety of research applications.NET is a key protein involved in the regulation of norepinephrine levels, impacting important physiological functions such as mood, stress response, and blood pressure control.
Dysregulation of NET activity has been linked to various neurological conditions and psychiatric disorders, emphasizing the importance of studying this protein as a potential therapeutic target.With its reliable performance and precise measurement capabilities, the Mouse NET ELISA Kit is a valuable resource for researchers seeking to understand the roles of NET in health and disease, as well as for developing novel treatment strategies.
Product Name:
Mouse Norepinephrine Transporter (NET) ELISA Kit
Product Code:
MODL00932
Size:
96 Assays
Target:
NET
Synonyms:
SLC6A2, NAT1, NET, NET1, Sodium-dependent noradrenaline transporter, Noradrenaline Transporter, Solute Carrier Family 6 member 2, Neurotransmitter transporter, Noradrenalin
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
