Mouse Nqo1 (NAD (P) H dehydrogenase [quinone] 1) ELISA Kit
The Mouse NQO1 (NAD(P)H Dehydrogenase Quinone 1) ELISA Kit is a highly reliable and sensitive assay designed for the accurate measurement of NQO1 levels in mouse serum, plasma, and cell lysates. This kit offers exceptional specificity and precision, ensuring consistent and reproducible results for a variety of experimental applications. NQO1 is a key enzyme involved in the detoxification of quinones and plays a crucial role in cellular antioxidant defense mechanisms. Dysregulation of NQO1 has been linked to oxidative stress, carcinogenesis, and neurodegenerative diseases, highlighting its significance as a biomarker for disease research and therapeutic development.
With the Mouse NQO1 ELISA Kit, researchers can easily quantify NQO1 expression levels in biological samples, allowing for the identification of potential biomarkers and therapeutic targets in various disease states. Its user-friendly format and robust performance make it an essential tool for studying the role of NQO1 in health and disease in mouse models.
Product Name:
Mouse Nqo1 (NAD (P) H dehydrogenase [quinone] 1) ELISA Kit
SKU:
AEFI00677
Reactivity:
Mouse
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
28.125 pg/mL
Range:
46.875-3000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-103%
85-100%
82-100%
EDTA Plasma (n = 5)
89-101%
84-99%
92-95%
Heparin Plasma (n = 5)
88-104%
82-96%
82-93%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-101
95
EDTA Plasma (n = 5)
86-99
93
Heparin Plasma (n = 5)
91-103
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
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