The Mouse NRG-1 (Neuregulin-1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of NRG-1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.Neuregulin-1 is a critical signaling protein that is involved in various cellular processes, including cell growth, migration, and differentiation. Dysregulation of NRG-1 has been linked to several diseases, including cancer, cardiovascular disorders, and neurological conditions.
As such, NRG-1 serves as a crucial biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Mouse NRG-1 CLIA Kit offers researchers a powerful tool for investigating the role of NRG-1 in health and disease, enabling advancements in basic research and potential clinical applications.
Product Name:
Mouse NRG-1 (Neuregulin 1) CLIA Kit
SKU:
MOES00078
Target:
Mouse NRG-1 (Neuregulin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NRG-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NRG-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NRG-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NRG-1. You can calculate the concentration of Mouse NRG-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
96-112
96-111
Average (%)
101
104
104
1:4
Range (%)
84-98
93-109
103-116
Average (%)
91
101
110
1:8
Range (%)
101-117
99-115
83-98
Average (%)
108
107
90
1:16
Range (%)
86-98
93-108
86-97
Average (%)
91
100
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
93-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.09
152.32
374.88
57.92
144.19
370.58
Standard deviation
6.82
11.03
36.7
6.24
12.43
27.9
C V (%)
12.85
7.24
9.79
10.77
8.62
7.53
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NRG-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NRG-1 in samples. No significant cross-reactivity or interference between Mouse NRG-1 and analogues was observed.
