The Mouse NSE (Neuron-Specific Enolase) ELISA Kit is a powerful tool for the precise measurement of NSE levels in mouse serum, plasma, and cell culture supernatants. With outstanding sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent choice for various research studies.NSE is a key enzyme found in neurons, playing a vital role in energy metabolism and serving as a marker for neuronal damage or injury. Monitoring NSE levels can provide valuable insights into neurological disorders, brain injuries, and neurodegenerative diseases, making this kit indispensable for researchers in the field of neuroscience.Get your hands on the Mouse NSE ELISA Kit today and unlock new possibilities for your research on brain health and neuronal function.
Product Name:
Mouse NSE (Neuron-Specific Enolase) ELISA Kit
SKU:
MOES00691
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
