The Mouse Osteopontin (OPN) ELISA Kit is meticulously designed to quantitatively detect Osteopontin levels in various mouse biological samples. Osteopontin, a multifunctional protein, is involved in several cellular processes including bone mineralization, immune responses, and inflammation modulation. This ELISA kit offers outstanding sensitivity and specificity, ensuring accurate and reproducible results in research investigations related to bone metabolism, immune regulation, and inflammatory conditions. Manufactured under stringent quality control measures, this kit provides reliable performance, making it an ideal choice for impactful scientific research.
Product Name:
Mouse OPN (Osteopontin) ELISA Kit
SKU:
AEES00225
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
