Description
Mouse PD-1 ELISA Kit
The Mouse PD-1 ELISA Kit is a sandwich enzyme-linked immunosorbent assay for the quantitative measurement of mouse PD-1 in serum, plasma and cell culture supernatants. With a sensitivity of 0.11 ng/mL and a detection range of 0.11-30 ng/mL, it delivers precise and reproducible quantification of mouse PD-1 for research applications.
The 96-well plate is coated with an antibody specific for mouse PD-1. PD-1 present in standards and samples is captured by the immobilized antibody and detected with a biotinylated anti-mouse PD-1 antibody, followed by HRP-conjugated streptavidin and TMB substrate. The Stop Solution changes the color from blue to yellow, and the intensity, read at 450 nm, is proportional to the amount of PD-1 bound.
| Product SKU: | SBRS7788 |
| Size: | 96T |
| Species: | Mouse |
| Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich |
| Method of Detection: | Colorimetric |
| Assay Type: | Quantitative |
| Sensitivity: | 0.11 ng/mL |
| Detection Range: | 0.11-30 ng/mL |
| Recommended Dilution: | 2 fold |
| Detection Wavelength: | 450 nm |
| Precision: | Intra-Assay CV: <10%; Inter-Assay CV: <12% |
| UniProt: | Q02242 |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see the technical manual. |
| Component | Quantity |
|---|---|
| Mouse PD-1 Microplate (96 wells, 12 x 8) | 1 plate |
| Mouse PD-1 Standard Protein | 2 vials |
| Mouse PD-1 Detection Antibody | 2 vials |
| Wash Buffer (20X) | 25 ml |
| HRP-Streptavidin (100X) | 200 µl |
| TMB One-Step Substrate Reagent | 12 ml |
| Stop Solution | 8 ml |
| Assay Diluent B (5X) | 15 ml |
| 96-Well Plate Sealing Film | 2 films |
- Prepare all reagents, samples and standards as instructed. Bring all reagents and samples to room temperature before use.
- Add 100 µl of each standard or sample to the appropriate wells.
- Incubate 2.5 hours at room temperature with gentle shaking.
- Wash 4 times with Wash Buffer (1X).
- Add 100 µl of prepared Detection Antibody to each well.
- Incubate 1 hour at room temperature with gentle shaking.
- Wash 4 times with Wash Buffer (1X).
- Add 100 µl of prepared HRP-Streptavidin solution to each well.
- Incubate 45 minutes at room temperature with gentle shaking.
- Wash 4 times with Wash Buffer (1X).
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 minutes at room temperature in the dark with gentle shaking.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
