The Mouse PDGFD (Platelet-Derived Growth Factor-D) CLIA Kit is a powerful tool for researchers looking to accurately measure PDGFD levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an invaluable asset for a variety of research applications.PDGFD is a key signaling protein that plays a critical role in promoting cell growth and division, as well as in regulating blood vessel formation.
Dysregulation of PDGFD has been implicated in various diseases, including cancer and cardiovascular disorders, highlighting its importance as a potential therapeutic target and biomarker.By utilizing the Mouse PDGFD CLIA Kit, researchers can gain valuable insights into the role of PDGFD in health and disease, paving the way for further advancements in understanding and treating these conditions.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGFD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PDGFD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDGFD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PDGFD. You can calculate the concentration of Mouse PDGFD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-116
88-100
96-109
Average (%)
106
93
101
1:4
Range (%)
97-111
91-103
87-97
Average (%)
103
97
92
1:8
Range (%)
94-107
85-96
89-105
Average (%)
102
91
96
1:16
Range (%)
101-113
97-114
86-100
Average (%)
107
104
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
99
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
99-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.85
69.81
186.36
24.83
63.7
172.54
Standard deviation
2.89
4.89
12.11
2.75
6.1
13.89
C V (%)
12.12
7.0
6.5
11.08
9.58
8.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PDGFD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PDGFD in samples. No significant cross-reactivity or interference between Mouse PDGFD and analogues was observed.
