Mouse Polyomavirus ELISA Kit is a valuable tool for researchers aiming to accurately quantify Polyomavirus levels in mouse samples such as serum, plasma, cell culture supernatants, and cell or tissue lysates. This kit offers high sensitivity and excellent specificity, ensuring precise and reproducible results for a variety of research applications. Mouse Polyomavirus (MPyV) is a small, non-enveloped DNA virus that has served as a foundational model for studying viral oncogenesis, tumour induction, and host-pathogen interactions. Infection with MPyV in susceptible mouse strains can lead to the development of multiple tumour types, and the virus encodes oncogenic proteins (large T, middle T, and small T antigens) that have been instrumental in elucidating key signalling pathways involved in cellular transformation, including those mediated by Src family kinases and PI3K/Akt. With the Mouse Polyomavirus ELISA Kit, researchers can confidently investigate viral replication, immune responses, and the molecular mechanisms of polyomavirus-induced tumourigenesis, supporting advances in virology, cancer biology, and immunology research.
Product Name:
Mouse Polyomavirus ELISA Kit
SKU:
AEFI04262
Reactivity:
Mouse
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-103%
90-103%
82-101%
EDTA Plasma (n = 5)
88-97%
82-100%
82-99%
Heparin Plasma (n = 5)
83-99%
84-98%
82-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-102
95
EDTA Plasma (n = 5)
91-104
99
Heparin Plasma (n = 5)
89-103
98
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent and Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10 to 20 minutes.
6
Stop Reaction and Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2 to 8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk and more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
