The Mouse PRF1 (Perforin-1) ELISA Kit is specifically designed for the accurate measurement of perforin-1 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it an invaluable tool for a variety of research applications.Perforin-1 is a key protein involved in the immune response, specifically in the cytotoxic activity of T and NK cells. It plays a crucial role in eliminating virally infected and transformed cells, highlighting its importance in immune surveillance and defense mechanisms. The measurement of perforin-1 levels can provide valuable insights into immune function and responses, making this ELISA kit essential for studying immune-related diseases and therapies.
Product Name:
Mouse PRF1 (Perforin 1) ELISA Kit
SKU:
MOES01347
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
