Mouse Protein Z Dependent Protease Inhibitor (ZPI) ELISA Kit
The Mouse Protein Z-Dependent Protease Inhibitor (ZPI) ELISA Kit is a cutting-edge immunological assay designed for the precise quantification of ZPI levels in mouse samples, including serum, plasma, and tissue lysates. This kit boasts exceptional sensitivity and specificity, allowing for accurate and reproducible results, making it an invaluable tool for a diverse array of experimental settings.ZPI is a key player in regulating blood coagulation and thrombus formation, serving as a crucial inhibitor of Factor XIa in the coagulation cascade.
Dysregulation of ZPI activity has been implicated in various pathological conditions, including thrombosis and hemostatic disorders, making it a pivotal target for research and clinical studies aimed at understanding and treating these diseases.By utilizing the Mouse Protein Z-Dependent Protease Inhibitor (ZPI) ELISA Kit, researchers can gain valuable insights into the role of ZPI in physiological and pathological processes, paving the way for the development of novel diagnostic tools and therapeutic interventions for hemostatic disorders.
Product Name:
Mouse Protein Z Dependent Protease Inhibitor (ZPI) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
