Description
Mouse RIGI Recombinant Protein (N-His)
Mouse RIGI Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn246-Val454, with an N-terminal His tag. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Antiviral innate immune response receptor RIG-I is a ~105 kDa protein. Innate immune receptor that senses cytoplasmic viral nucleic acids and activates a downstream signaling cascade leading to the production of type I interferons and pro-inflammatory cytokines. For research use only.
Forms a ribonucleoprotein complex with viral RNAs on which it homooligomerizes to form filaments. The homooligomerization allows the recruitment of RNF135 an E3 ubiquitin-protein ligase that activates and amplifies the RIG-I-mediated antiviral signaling in an RNA length-dependent manner through ubiquitination-dependent and -independent mechanisms. Upon activation, associates with mitochondria antiviral signaling protein (MAVS/IPS1) that activates the IKK-related kinases TBK1 and IKBKE which in turn phosphorylate the interferon regulatory factors IRF3 and IRF7, activating transcription of antiviral immunological genes including the IFN-alpha and IFN-beta interferons. Ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length).
| Product Name: | Mouse RIGI Recombinant Protein (N-His) |
| SKU: | GXRP13966 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mouse |
| Synonyms: | ATP-dependent RNA helicase DDX58, Antiviral innate immune response receptor RIG-I, DEAD box protein 58, Ddx58, EC:3.6.4.13, RIG-1, RIG-I, RIG-I-like receptor 1, RLR-1, RNA sensor RIG-I, Retinoic acid-inducible gene 1 protein, Retinoic acid-inducible gene I protein, Rigi |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asn246-Val454 |
| UniProt: | Q6Q899 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
