Description
Mouse RIPK3 Recombinant Protein (N-His)
Mouse RIPK3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Leu320, with an N-terminal His tag. Predicted molecular weight: 37.49 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Receptor-interacting serine/threonine-protein kinase 3 is a ~53 kDa protein. Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death. For research use only.
Necroptosis, a programmed cell death process in response to death-inducing TNF family members, is triggered by RIPK3 following activation by ZBP1. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity.
| Product Name: | Mouse RIPK3 Recombinant Protein (N-His) |
| SKU: | GXRP09863 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mouse |
| Synonyms: | Receptor-interacting serine/threonine-protein kinase 3; 2.7.11.1; RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3; mRIP3; Ripk3; Rip3 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Leu320 |
| Calculated MW: | 37.49 kDa |
| UniProt: | Q9QZL0 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
