Mouse S100A8 (S100 Calcium Binding Protein A8) ELISA Kit
The Mouse S100 Calcium Binding Protein A8 (S100A8) ELISA Kit is a specialized assay crafted to quantitatively analyze the levels of S100A8 in diverse biological samples with precision and high sensitivity. S100A8, a member of the S100 protein family, is a calcium-binding protein known for its involvement in various cellular processes, including inflammation, immune response modulation, and cell differentiation. High levels of S100A8 are often linked to inflammatory conditions, autoimmune diseases, cancer progression, and other pathological states, making it a crucial protein of interest in biomedical research. By accurately measuring S100A8 levels, researchers can unravel its intricate roles in inflammation, immunity, and disease pathogenesis, providing valuable insights into potential biomarkers and therapeutic targets. Understanding the functions and regulation of S100A8 is essential for deciphering its impact on cellular dynamics and its contribution to disease development, offering new avenues for therapeutic interventions and diagnostic strategies. The Mouse S100A8 ELISA Kit from Assay Genie is meticulously designed to deliver exceptional sensitivity and specificity, ensuring robust and reproducible results for your investigative studies. Developed under strict quality control measures, this kit guarantees reliable performance and user-friendly procedures, making it a trusted tool for researchers investigating the biological roles of S100A8 in health and disease. Choose Assay Genie's S100A8 ELISA Kit for precise quantification of S100A8 levels and illuminate the complexity of S100A8-mediated processes in diverse biological contexts, ranging from inflammation to cancer research and beyond.
Product Name:
Mouse S100A8 (S100 Calcium Binding Protein A8) ELISA Kit
SKU:
AEES00241
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
