Mouse S100A9 (S100 Calcium Binding Protein A9) ELISA Kit
The Mouse S100 Calcium Binding Protein A9 (S100A9) ELISA Kit is a specialized assay designed for the precise quantitative analysis of S100A9 levels in a variety of biological samples. S100A9, a member of the S100 protein family, is a calcium-binding protein known for its involvement in various biological processes, including inflammation, immune response regulation, and cell differentiation. Elevated levels of S100A9 are often associated with inflammatory conditions, autoimmune disorders, and cancer progression, making it a vital protein of interest in diverse research areas. Accurate measurement of S100A9 is essential for unraveling its role in modulating immune responses, understanding disease pathogenesis, and potentially identifying diagnostic or therapeutic targets. By quantifying S100A9 levels in biological samples, researchers can gain valuable insights into the protein's involvement in inflammatory pathways, cell signaling mechanisms, and disease progression. This quantitative data is pivotal for advancing our knowledge of S100A9 biology and its implications in various health conditions. The Mouse S100A9 ELISA Kit from Assay Genie offers high sensitivity, specificity, and reproducibility, ensuring dependable and consistent results for your research needs. Manufactured under rigorous quality control standards, this kit provides robust performance and user-friendly protocols, making it an ideal choice for scientists investigating the functional roles and clinical significance of S100A9. Choose Assay Genie's S100A9 ELISA Kit for accurate quantification of S100A9 levels and uncover the multifaceted roles of S100A9 in inflammation, immunity, and disease processes.
Product Name:
Mouse S100A9 (S100 Calcium Binding Protein A9) ELISA Kit
SKU:
AEES00242
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
