The Mouse SCF (Stem Cell Factor) ELISA Kit is a highly sensitive and specific assay designed for the precise detection of SCF levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it ideal for various research applications.Stem Cell Factor (SCF) is a critical cytokine that plays a key role in regulating hematopoiesis, cell survival, and proliferation. It is essential for the maintenance and expansion of hematopoietic stem cells, making it an important factor in stem cell research and regenerative medicine.With its high sensitivity and specificity, the Mouse SCF ELISA Kit is a valuable tool for studying the role of SCF in various biological processes and diseases, facilitating research in areas such as stem cell biology, cancer, and immune system regulation.
Product Name:
Mouse SCF (Stem Cell Factor) ELISA Kit
SKU:
MOES01144
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
