The Mouse SELP (P-Selectin) ELISA Kit is specifically designed for the precise measurement of P-Selectin levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, providing accurate and consistent results for various research purposes.P-Selectin is a key adhesion molecule involved in inflammation and immune responses, playing a crucial role in cell adhesion and migration. It is implicated in various pathological conditions such as cardiovascular diseases, inflammatory disorders, and thrombosis, making it a valuable biomarker for studying these diseases and developing novel therapeutic interventions. Overall, the Mouse SELP (P-Selectin) ELISA Kit is an essential tool for researchers investigating the role of P-Selectin in disease pathogenesis and for identifying potential targets for therapeutic interventions.
Product Name:
Mouse SELP (P-Selectin) ELISA Kit
SKU:
MOES01146
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
