The Mouse Semaphorin 3A (Sema3A) ELISA Kit is a powerful tool for measuring levels of Sema3A in mouse serum, plasma, and tissue culture samples. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it well-suited for a variety of research applications. Sema3A is a key member of the semaphorin family of proteins that play a critical role in regulating neuronal development, immune responses, and angiogenesis. Dysregulation of Sema3A has been implicated in a range of disorders, including cancer, autoimmune diseases, and neurological conditions, making it a valuable target for therapeutic interventions.
By detecting and quantifying Sema3A levels, researchers can gain valuable insights into its role in disease pathogenesis and identify potential avenues for novel treatment strategies. The Mouse Semaphorin 3A ELISA Kit offers a reliable and efficient solution for studying the function of this important protein in a preclinical mouse model system.
Product Name:
Mouse Semaphorin 3A/SEMA3A ELISA Kit
SKU:
MOFI00558
Reactivity:
Mouse
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
9.375 pg/mL
Range:
15.625-1000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
99-104%
87-98%
82-96%
EDTA Plasma (n = 5)
82-97%
85-99%
89-99%
Heparin Plasma (n = 5)
89-103%
82-99%
82-101%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-99
95
EDTA Plasma (n = 5)
85-100
97
Heparin Plasma (n = 5)
88-103
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
