Mouse SP-D (Pulmonary Surfactant Associated Protein D) ELISA Kit
The Mouse SP-D (Pulmonary Surfactant-Associated Protein D) ELISA Kit is specifically designed for the accurate measurement of SP-D levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications. SP-D is a key protein involved in immune defense in the lungs, playing a critical role in the innate immune response and surfactant function. Dysregulation of SP-D has been associated with various respiratory diseases, including asthma, chronic obstructive pulmonary disease (COPD), and pulmonary fibrosis, making it a valuable biomarker for investigating these conditions and developing potential therapies. By utilizing the Mouse SP-D ELISA Kit, researchers can gain valuable insights into the role of SP-D in respiratory health and disease, advancing our understanding of lung biology and potential treatment options for respiratory conditions.
Product Name:
Mouse SP-D (Pulmonary Surfactant Associated Protein D) ELISA Kit
SKU:
MOES01432
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
