Mouse Succinate Dehydrogenase Complex Subunit D (SDHD) ELISA Kit
The Mouse Succinate Dehydrogenase Complex Subunit D (SDHD) ELISA Kit is a powerful tool for detecting levels of SDHD in mouse samples. This kit offers high sensitivity and specificity, providing accurate and reproducible results for researchers studying mitochondrial function and metabolism.SDHD is a key component of the succinate dehydrogenase complex, playing a crucial role in the electron transport chain and energy production in mitochondria.
Dysregulation of SDHD has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic disorders.By accurately measuring SDHD levels in mouse samples, researchers can gain valuable insights into mitochondrial function and its implications in disease pathology. The Mouse SDHD ELISA Kit is a valuable asset for studies investigating mitochondrial dysfunction and its impact on health and disease.
Product Name:
Mouse Succinate Dehydrogenase Complex Subunit D (SDHD) ELISA Kit
Product Code:
MODL01165
Size:
96 Assays
Target:
SDHD
Synonyms:
PGL, PGL1, QPs3, Succinate-ubiquinone reductase membrane anchor subunit, Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
