The Mouse Sulfatase 2 (Sulf2) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of Sulfatase 2 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.Sulfatase 2 is an important enzyme involved in the regulation of sulfate homeostasis and the modification of sulfated molecules. It plays a crucial role in various physiological processes, including cell signaling, growth factor activation, and extracellular matrix remodeling.
Dysregulation of Sulfatase 2 has been implicated in numerous diseases, such as cancer, metabolic disorders, and developmental abnormalities, highlighting its significance as a biomarker for disease detection and therapeutic development.Overall, the Mouse Sulfatase 2 (Sulf2) ELISA Kit is a valuable tool for studying the role of Sulfatase 2 in health and disease, providing researchers with accurate and dependable results for their experiments.
Product Name:
Mouse Sulfatase 2 (SULF2) ELISA Kit
Product Code:
MODL01243
Size:
96 Assays
Target:
SULF2
Synonyms:
HSULF-2, Extracellular sulfatase Sulf-2
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
