Mouse Synaptosomal Associated Protein 25kDa (SNAP25) ELISA Kit
The Mouse Synaptosomal-Associated Protein 25kDa (SNAP25) ELISA Kit is designed for the precise quantification of SNAP25 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of experimental studies.SNAP25 is a key protein involved in neurotransmission, specifically in the release of neurotransmitters at synapses. Dysregulation of SNAP25 has been implicated in various neurological disorders, including schizophrenia, ADHD, and Alzheimer's disease.
This ELISA kit provides researchers with a valuable tool for investigating the role of SNAP25 in these conditions and exploring potential therapeutic interventions.With its user-friendly protocol and fast assay time, the Mouse SNAP25 ELISA Kit is a valuable asset for researchers interested in understanding the molecular mechanisms underlying neurological diseases and developing novel treatment strategies. Order yours today to advance your research in this exciting field.
Product Name:
Mouse Synaptosomal Associated Protein 25kDa (SNAP25) ELISA Kit
Product Code:
MODL01208
Size:
96 Assays
Target:
SNAP25
Synonyms:
RIC4, SEC9, SNAP, SUP, Super protein, Resistance To Inhibitors Of Cholinesterase 4 Homolog
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
