The Mouse TF (Tissue Factor) ELISA Kit is specifically designed for the precise measurement of tissue factor levels in mouse samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it ideal for various research studies and applications.Tissue factor, also known as thromboplastin, is a key protein involved in blood coagulation and clotting. Dysregulation of tissue factor expression has been linked to various diseases such as thrombosis, inflammation, and cancer. Therefore, monitoring tissue factor levels can provide valuable insights into these pathological conditions and aid in the development of potential therapeutic strategies.Overall, the Mouse TF ELISA Kit offers a reliable tool for researchers interested in studying the role of tissue factor in health and disease, helping advance our understanding of coagulation disorders and related disorders.
Product Name:
Mouse TF (Tissue factor) ELISA Kit
SKU:
MOES01565
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
87-100
97-113
96-109
Average (%)
94
104
101
1:4
Range (%)
90-103
93-106
95-111
Average (%)
97
100
102
1:8
Range (%)
90-103
96-111
98-112
Average (%)
96
102
104
1:16
Range (%)
89-102
96-109
90-107
Average (%)
96
103
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
95-108
102
Cell Culture Media (n=8)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
18.26
54.87
147.88
19.13
58.19
140.84
Standard deviation
0.9
2.81
10.23
1.16
4.18
12.56
C V (%)
4.93
5.13
6.92
6.07
7.19
8.92
Uniport ID:
P20352
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Mouse TF in samples. No significant cross-reactivity or interference between Mouse TF and analogues was observed
Storage:
2-8℃
Research Area:
Cancer,Cardiovascular
Wolff et al.
Prophylaxis with abemaciclib delays tumorigenesis in dMMR mice by altering immune responses and reducing immunosuppressive extracellular vesicle secretion
