The Mouse Thy1 Cell Surface Antigen (Thy1) ELISA Kit is a powerful tool for detecting and quantifying levels of Thy1 in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Thy1 is a cell surface glycoprotein that plays a key role in cell adhesion, migration, and signal transduction. It is commonly used as a marker for various cell types, including T cells, neurons, and fibroblasts.
Dysregulation of Thy1 expression has been implicated in inflammatory diseases, cancer, and neurological disorders, highlighting its importance as a potential therapeutic target.With easy-to-follow protocols and quick assay times, the Mouse Thy1 Cell Surface Antigen (Thy1) ELISA Kit is an essential tool for researchers studying the role of Thy1 in health and disease. Order yours today to advance your research and uncover new insights into cellular function and pathology.
Product Name:
Mouse Thy1 Cell Surface Antigen (Thy1) ELISA Kit
Product Code:
MODL01281
Size:
96 Assays
Target:
Thy1
Synonyms:
CD90, CDw90, THY mocyte differentiation antigen 1
Detection Method:
Sandwich
Range:
12.5-800pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
