Mouse TNF-beta/LTA/TNFSF1B PharmaGenie ELISA Kit (SBRS1543)

SKU:
SBRS1543
Product type:
ELISA
Reactivity:
Mouse
Application:
ELISA
ELISA Type:
Sandwich
€665

Description

Mouse TNF-beta/LTA/TNFSF1B PharmaGenie ELISA - Kit Data

Product SKU:SBRS1543
Size:96T
Application:This ELISA antibody pair detects Mouse TNF-beta. Other species not determined.
Uniprot:P09225
Gene ID:16992
Gene Names:LTA / TNFB / TNFSF1
Synonyms:Lymphotoxin-alpha (LT-alpha) (TNF-beta) (Tumor necrosis factor ligand superfamily member 1)
Target Species:Mouse
Compatible Sample Types:Cell Culture Supernatants, Plasma, Serum
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.43 ng/ml - 100 ng/ml
Sensitivity:0.43 ng/ml
Recommended Dilution:2 fold
Storage/Stability:The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Stop Solution
4.Assay Diluent(s)
5.Lyophilized Standard
6.Biotinylated Detection Antibody
7.Streptavidin-Conjugated HRP
8.TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Mouse TNF-beta/LTA/TNFSF1B ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Kit Protocol:

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 ul of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 ul of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.
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