Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance.Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.
< 1.0 EU/mg of the protein as determined by the LAL method
Purity:
> 90% as determined by reducing SDS-PAGE.
Calculated MW:
20.3 kDa
Observed MW:
40-50 kDa
Bio-Activity:
Not validated for activity
Form:
Lyophilized powder
Source:
HEK293 Cells-derived Mouse TNFα protein Gly57-Leu235, with an C-terminal His
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Storage:
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to - 80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
