The Mouse Transforming Growth Factor Beta-1 (TGFb1) ELISA Kit is a powerful tool designed for accurate and precise measurement of TGFb1 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.TGFb1 is a key cytokine involved in regulating cell growth, differentiation, and immune response. It plays a crucial role in numerous biological processes, including tissue regeneration, wound healing, and immune system modulation.
Dysregulation of TGFb1 has been implicated in various diseases such as cancer, fibrosis, and autoimmune disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse TGFb1 ELISA Kit provides researchers with a reliable tool to accurately quantify TGFb1 levels in mouse samples, enabling in-depth investigations into the role of TGFb1 in various physiological and pathological processes.
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
