Mouse Transient Receptor Potential Cation Channel Subfamily M, Member 6 (TRPM6) ELISA Kit
The Mouse Transient Receptor Potential Cation Channel Subfamily M Member 6 (TRPM6) ELISA Kit is a powerful tool designed for the accurate quantification of TRPM6 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results that are crucial for a variety of research applications.TRPM6 is a key member of the TRP ion channel family, responsible for regulating magnesium homeostasis and calcium signaling in cells. Dysregulation of TRPM6 has been linked to various diseases and disorders, including hypomagnesemia, cardiovascular conditions, and neurological disorders.
As such, TRPM6 serves as an important biomarker for understanding these conditions and developing potential therapeutic interventions.With its high-quality components and user-friendly protocol, the Mouse TRPM6 ELISA Kit is an essential tool for researchers studying the roles of TRPM6 in health and disease. Invest in this kit today to advance your research and uncover new insights into the complex mechanisms of TRP ion channels.
Product Name:
Mouse Transient Receptor Potential Cation Channel Subfamily M, Member 6 (TRPM6) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
