Mouse TREM-2 PharmaGenie ELISA Kit (SBRS1550)
- SKU:
- SBRS1550
- Product type:
- ELISA Kit
- Reactivity:
- Mouse
- Application:
- ELISA
- ELISA Type:
- Sandwich
Frequently bought together:
Description
system_update_altDatasheet
Mouse TREM-2 PharmaGenie ELISA - Kit Data
| Product SKU: | SBRS1550 |
| Size: | 96T |
| Application: | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse Ret, RGM-B, RGM-C, ROBO3, SEMA3C, SEMA3F, SEMA4C, SEMA4F, SEMA4G, SEMA6C, Siglec-3, Siglec-E, SIGNR1, Slit2, SMOC-1, SorCS2, SP-D, SR-AI, STC-2, Syndecan-1, Syndecan-3, TFPI-2, TGF-beta RI, TGF-beta RII, TGM2, TIGIT, TIM-3, TLR2, TNFRH3, Tpo R, TRAIL R2, TrkC, TROP-2, Trypsin 3, Tryptase-5, TSLP R, uPAR, VE-Cadherin, Wnt-2b. |
| Uniprot: | Q99NH8 |
| Gene ID: | 83433 |
| Gene Names: | Trem2 / Trem2a / Trem2b / Trem2c |
| Synonyms: | Triggering receptor expressed on myeloid cells 2 (TREM-2) (Triggering receptor expressed on monocytes 2) |
| Target Species: | Mouse |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.034 ng/ml -25 ng/ml |
| Sensitivity: | 0.034 ng/ml |
| Recommended Dilution: | 2 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
Kit Components:
| 1. | Pre-Coated 96-well Strip Microplate |
| 2. | Wash Buffer |
| 3. | Stop Solution |
| 4. | Assay Diluent(s) |
| 5. | Lyophilized Standard |
| 6. | Biotinylated Detection Antibody |
| 7. | Streptavidin-Conjugated HRP |
| 8. | TMB One-Step Substrate |
Other materials and equipment required:
The Assay Genie Mouse TREM-2 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Kit Protocol:
| 1. | Prepare all reagents, samples and standards as instructed in the manual. |
| 2. | Add 100 ul of standard or sample to each well. |
| 3. | Incubate 2.5 h at RT or O/N at 4°C. |
| 4. | Add 100 ul of prepared biotin antibody to each well. |
| 5. | Incubate 1 h at RT. |
| 6. | Add 100 ul of prepared Streptavidin solution to each well. |
| 7. | Incubate 45 min at RT. |
| 8. | Add 100 ul of TMB One-Step Substrate Reagent to each well. |
| 9. | Incubate 30 min at RT. |
| 10. | Add 50 ul of Stop Solution to each well. |
| 11. | Read at 450 nm immediately. |
