The Mouse TRF (Transferrin) ELISA Kit is specifically designed for the precise measurement of transferrin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Transferrin is a key protein involved in iron transport and homeostasis, crucial for various physiological processes such as oxygen transport and cell growth. Dysregulation of transferrin levels has been implicated in diseases like iron deficiency anemia and hemochromatosis, highlighting its importance as a biomarker in studying these conditions and developing potential therapeutic interventions. With its reliability and performance, the Mouse TRF (Transferrin) ELISA Kit is an invaluable tool for researchers studying iron metabolism, iron-related disorders, and therapeutic strategies targeting transferrin levels. Get yours today to advance your research with confidence.
Product Name:
Mouse TRF (Transferrin) ELISA Kit
SKU:
MOES01577
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
