Mouse Troponin I Type 2, Fast Skeletal (TNNI2) ELISA Kit
The Mouse Troponin I Type 2 (fast skeletal) ELISA Kit is specifically designed for the accurate and precise measurement of troponin I levels in mouse serum, plasma, and cell lysates. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research experiments.Troponin I Type 2 is a critical component of the fast skeletal muscle contraction process, playing a crucial role in muscle function and performance. Abnormal levels of troponin I have been linked to muscle disorders, heart diseases, and other conditions, making it a valuable biomarker for studying these diseases and developing potential treatments.
With its user-friendly protocol and high-quality components, the Mouse Troponin I Type 2 ELISA Kit is a valuable tool for researchers in the fields of muscle biology, cardiovascular research, and drug development. Trust in this kit to deliver accurate and reliable results for your experiments.
Product Name:
Mouse Troponin I Type 2, Fast Skeletal (TNNI2) ELISA Kit
Product Code:
MODL01308
Size:
96 Assays
Target:
TNNI2
Synonyms:
AMCD2B, DA2B, FSSV, Troponin I Fast Twitch 2, Troponin I, fast-twitch isoform
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
