The Mouse Utrophin (UTRN) ELISA Kit is a reliable and accurate tool for detecting utrophin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides precise and reproducible results, making it ideal for various research applications.Utrophin is an important protein involved in maintaining muscle integrity and function. It serves as a functional analog of dystrophin, playing a crucial role in muscle repair and regeneration. Dysregulation of utrophin expression has been implicated in muscular dystrophies and other muscle-related disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
Overall, the Mouse Utrophin (UTRN) ELISA Kit is a valuable tool for researchers interested in studying muscle biology, muscular dystrophies, and related disorders in mouse models. Its high performance and reliability make it a valuable asset for advancing our understanding of utrophin biology and developing novel treatment strategies.
Product Name:
Mouse Utrophin (UTRN) ELISA Kit
Product Code:
MODL01365
Size:
96 Assays
Target:
UTRN
Synonyms:
DRP1, DMDL, DRP, Homologous To Dystrophin, Dystrophin-related protein 1
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
