The Mouse VWF (Von Willebrand Factor) ELISA Kit is specifically designed for the precise quantification of Von Willebrand Factor levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Von Willebrand Factor is a key protein involved in blood clotting and platelet adhesion, playing a critical role in hemostasis. Dysregulation of VWF levels is associated with various bleeding disorders, making it an important biomarker for studying these conditions and developing therapeutic interventions.With the Mouse VWF ELISA Kit, researchers can easily measure VWF levels in mouse samples with confidence and precision, advancing our understanding of hemostasis and contributing to the development of potential treatments for bleeding disorders.
Product Name:
Mouse vWF (von Willebrand Factor) ELISA Kit
SKU:
MOES01614
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
