Mouse Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit
The Mouse Wingless-Type MMTV Integration Site Family Member 5A (Wnt5A) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of Wnt5A levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.Wnt5A is a key signaling molecule involved in various biological processes, including embryonic development, tissue homeostasis, and disease progression. Dysregulation of Wnt5A signaling has been implicated in a variety of conditions, such as cancer, inflammatory diseases, and developmental disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its high performance and versatility, the Mouse Wnt5A ELISA Kit is an essential tool for researchers seeking to investigate the role of Wnt5A in health and disease, ultimately advancing our understanding of this important signaling pathway.
Product Name:
Mouse Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit
Product Code:
MODL01400
Size:
96 Assays
Target:
WNT5A
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
