The Mouse X-Box Binding Protein 1 (XBP1) ELISA Kit is specifically designed for the accurate measurement of XBP1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and reliable results for a variety of research applications.XBP1 is a key transcription factor involved in the regulation of the unfolded protein response (UPR), which plays a critical role in cellular stress responses and maintaining protein homeostasis. Dysregulation of XBP1 has been implicated in various disease states, including cancer, neurodegenerative disorders, and autoimmune diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic strategies.
With its advanced technology and robust performance, the Mouse XBP1 ELISA Kit is an essential tool for researchers looking to investigate the role of XBP1 in disease pathogenesis and develop novel treatment approaches. Trust in this kit to deliver accurate and reproducible results for your research needs.
Product Name:
Mouse X-Box Binding Protein 1 (XBP1) ELISA Kit
Product Code:
MODL01401
Size:
96 Assays
Target:
XBP1
Synonyms:
TREB5, XBP2, Tax-responsive element-binding protein 5
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
