SynonymsKARP1,KU80,KUB2,Ku86,NFIV,TLAA,Lupus Ku Autoantigen Protein P80,Nuclear factor IV,86 kDa of Ku antigen,CTC box-binding factor 85 kDa,Thyroid-lupus autoantigen
ReactivityMouse
Sample TypeTissue Homogenates And Other Biological Fluids
SKU:
MODL01404
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27641
Sensitivity:
0.072ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
KARP1,KU80,KUB2,Ku86,NFIV,TLAA,Lupus Ku Autoantigen Protein P80,Nuclear factor IV,86 kDa of Ku antigen,CTC box-binding factor 85 kDa,Thyroid-lupus autoantigen
The Mouse X-ray Repair Cross Complementing 5 (XRCC5) ELISA Kit is a valuable tool for researchers studying DNA repair processes in mice. This kit is designed for accurate detection of XRCC5 levels in mouse serum, plasma, and cell culture supernatants, providing high sensitivity and specificity for reliable results.XRCC5 is a key protein involved in DNA double-strand break repair and maintenance of genome stability.
Dysregulation of XRCC5 has been linked to various diseases, including cancer and genetic disorders, making it a crucial biomarker for understanding disease mechanisms and potential therapeutic targets.With its easy-to-use format and robust performance, the Mouse XRCC5 ELISA Kit is an essential tool for researchers studying DNA repair pathways and exploring new avenues in disease research and drug development.
KARP1, KU80, KUB2, Ku86, NFIV, TLAA, Lupus Ku Autoantigen Protein P80, Nuclear factor IV, 86 kDa of Ku antigen, CTC box-binding factor 85 kDa, Thyroid-lupus autoantigen
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
