Description
TPO-R/CD110/c-Mpl Monoclonal Antibody (CAB19729)
The TPO-R/CD110/c-Mpl Monoclonal Antibody (CAB19729) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various research applications, including Western blot analysis.CD110 (c-Mpl) is a receptor for thrombopoietin, a cytokine involved in the production of platelets and regulation of hematopoietic stem cells. Dysregulation of the CD110 (c-Mpl) pathway has been implicated in various hematologic disorders, making it a key target for investigation in the fields of hematology and oncology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | TPO-R/CD110/c-Mpl Monoclonal Antibody |
| SKU: | CAB19729 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Clone Number: | ARC2257 |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | HRVL GQYL RDTA ALSP PKAT VSDT CEEV EPSL LEIL PKSS ERTP LPLC SSQA QMDY RRLQ PSCL GTMP LSVC PPMA ESGS CCTT HIAN HSYL PLSY WQQP | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | MPLV, TPOR, C-MPL, CD110, THPOR, THCYT2, TPO-R/CD110/c-Mpl |
| Positive Sample: | A-549, Raji, THP-1, Mouse heart, Rat liver |
| Cellular Localization: | Cell Surface, External Side Of Plasma Membrane, Golgi Apparatus, Nuclear Membrane, Plasma Membrane. |
| Calculated MW: | 71kDa |
| Observed MW: | 80kDa |
In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
| Purification Method | Affinity purification |
| Gene ID | 4352 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using TPO-R/CD110/c-Mpl Rabbit mAb (CAB19729) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s. |
 | Western blot analysis of various lysates using TPO-R/CD110/c-Mpl Rabbit mAb (CAB19729) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s. |
