Description
Monkeypox Virus (MPXV) A30L Protein IgM ELISA Kit
The Monkeypox Virus A30L Protein Human IgM ELISA Kit is a reliable and accurate tool for the detection of IgM antibodies specific to the A30L protein of the Monkeypox virus in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring trustworthy and reproducible results for a variety of research applications.The A30L protein is a key component of the Monkeypox virus, playing a crucial role in the virus' pathogenesis and immune response.
By detecting IgM antibodies against this protein, researchers can gain valuable insights into the immune response to Monkeypox virus infection, aiding in the development of diagnostic tools and potential therapeutics.Overall, the Monkeypox Virus A30L Protein Human IgM ELISA Kit is a valuable resource for studying Monkeypox virus infections and advancing research efforts in the field of virology and infectious diseases.
| Product Name: | Monkeypox Virus (MPXV) A30L Protein IgM ELISA Kit |
| Product Code: | MKRS0007 |
| Species Detected: | Human |
| Gene Symbols: | IGM |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
