Description
Monkeypox Virus (MPXV) A35R Protein IgG ELISA Kit
The Monkeypox Virus A35R Protein Human IgG ELISA Kit is a specialized assay designed for the accurate and sensitive detection of human IgG antibodies against the A35R protein of the Monkeypox virus. This kit is ideal for researchers studying Monkeypox infection, immunity, and vaccine development.The kit features high specificity and reliability, providing reproducible results for serum, plasma, and cell culture supernatants. The Monkeypox virus is a zoonotic disease that poses a threat to human health, making the detection of specific antibodies crucial for understanding the immune response to the virus.
By utilizing this ELISA kit, researchers can gain valuable insights into the immune response against Monkeypox virus infection and develop potential therapeutics and vaccines. The Monkeypox Virus A35R Protein Human IgG ELISA Kit is a valuable tool for studying infectious diseases and advancing research in virology.
| Product Name: | Monkeypox Virus (MPXV) A35R Protein IgG ELISA Kit |
| Product Code: | MKRS0012 |
| Species Detected: | Human |
| Gene Symbols: | IGG |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
