Description
Monkeypox Virus (MPXV) H3L Protein IgG ELISA Kit
The Monkeypox Virus H3L Protein Human IgG ELISA Kit is specifically designed for the accurate detection of IgG antibodies against the H3L protein of the Monkeypox virus in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.The H3L protein is a key antigen involved in the immune response against Monkeypox virus infection.
By detecting IgG antibodies against this protein, researchers can gain valuable insights into the immune response to Monkeypox virus and its potential implications for vaccine development and therapeutic interventions.Overall, the Monkeypox Virus H3L Protein Human IgG ELISA Kit provides a valuable tool for studying Monkeypox virus infection and advancing our understanding of the immune response to this emerging viral pathogen.
| Product Name: | Monkeypox Virus (MPXV) H3L Protein IgG ELISA Kit |
| Product Code: | MKRS0027 |
| Species Detected: | Human |
| Gene Symbols: | IGG |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
