Description
Monkeypox Virus (MPXV) I1L Protein IgM ELISA Kit
The Monkeypox Virus I1L Protein Human IgM ELISA Kit is a specialized assay designed for the accurate and sensitive detection of IgM antibodies against the I1L protein of the Monkeypox virus in human serum and plasma samples. With high specificity and precision, this kit provides reliable results for research and diagnostic purposes.The I1L protein is a key component of the Monkeypox virus and plays a crucial role in the pathogenesis of the disease.
Detection of IgM antibodies against this protein can aid in the early diagnosis of Monkeypox virus infection and monitoring of immune response.This ELISA kit offers a simple and efficient method for studying Monkeypox virus infections, vaccine development, and epidemiological studies. It is a valuable tool for researchers and healthcare professionals working in the field of virology and infectious diseases.
| Product Name: | Monkeypox Virus (MPXV) I1L Protein IgM ELISA Kit |
| Product Code: | MKRS0031 |
| Species Detected: | Human |
| Gene Symbols: | IGM |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
